Edu detectpro cell proliferation flow cytometry kits. Cell proliferation elisa, brdu colorimetric y version 17 content version. Because the cells are frozen, samples can stored for up to 4 weeks and run in batches, allowing the. These asbestos materials are typical of the asbestos found in bulk samples during routine asbestos inspections of building materials. Safety data sheet sds osha hazcom standard 29 cfr 1910. Flammability of the product autoignition temperature not available. Because brdu can replace thymidine during dna replication, it can cause. Working with bromodeoxyuridine brdu ohs information. Exposure assessment of laboratory workers to hazardous waste. View or download the brdu cell proliferation assay msds material safety data sheet or sds for qia58 from merck. Simply remove the culture media, freeze and lyse cells, then read fluorescence on a standard microplate reader with a fluorescein filter. Wear government approved airpurifying respirator if needed.
The kit contains sufficient material for 2 x 96 well plate assays. The halogenated pyrimidine thymidine analog bromodeoxyuridine brdu is. These products are carbon oxides co, co2, and other toxic compounds nitrogen. Brdu is a thymidine analog, incorporated into cell nuclei during dna synthesis prior to mitosis. Msds examples sds sheets msds authoring services inc. Cells can naturally incorporate the thymidine analog brdu into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Uses advised against this product is not intended for use in diagnostic procedures or therapeutics. Examples safety data sheet sdsmsds here you can see examples of safety data sheets in some of the various languages we handle.
Sigmaaldrich 207888 page 1 of 8 sigmaaldrich material safety data sheet version 5. Under the new ghs, globally harmonized system, the name has been changed from msds, material safety data sheet, and simplified to sds, safety data sheet. Brdu is commonly used in the detection of proliferating cells in living. Page 111 safety data sheet sds osha hazcom standard 29 cfr 1910. Certificate of analysis material safety data sheet. Brdu is also incorporated into bone marrow cells in culture at a final concentration of 10 m at 37 c for one hour. Apoptosis,dna,damage,and,cell,proliferation,kit,ruo 562253. Brdu cell proliferation assay msds material safety data sheet or sds, coa and coq, dossiers, brochures and other available documents. We work hard to ensure that we are fully uptodate with regulations, and make sure to incorporate changes as soon as they occur, so that you do not risk having outofdate safety data sheets. This incubation period is required for equilibration of dyedna binding, resulting in a stable. Brdu is commonly used in the detection of proliferating cells in living tissues and works by substituting for thymidine during dna replication thereby incorporating itself into the newly synthesized dna.
No special precautions are necessary if used correctly. Edu detectpro cell proliferation flow cytometry kits user manual version 1. Identification product identifier product number 7016l product name dtt dithiothreitol recommended use of the chemical and restrictions on use identified uses this product is intended for. Useful for the study of dna synthesis where it is incorporated into dna in place of thymidine. Therefore samples have to be subjected to harsh denaturing conditions resulting in degradation of the structure of the specimen.
Opadry ag colorcon 415 moyer boulevard west point, pa 19486 usa email. Analysis of incorporated brdu can be with an anti brdu antibody. Fluorescence histograms of eduincorporation with baseclick edu flow cytometry kit. Edu detectpro cell proliferation hts kits user manual 301512. View or download the brdu cell proliferation assay, hts msds material safety data sheet or sds for hts01 from merck. Product identifier product number 7002 product name stop solution reach registration number this substancemixture contains only ingredients which have been registered. August 2018 colorimetric immunoassay for the quantific ation of cell proliferation, based on the measurement of brdu. Toxicological properties health hazard information chlorine. Safety data sheet sds according to the reach regulation ec no. Apoptosis,dna,damage,and,cell,proliferation,kit,ruo. Safety glasses are recommended to prevent particulate matter from entering eyes while grinding or machining. Working with bromodeoxyuridine brdu ohs information sheet. Also, long sleeve cotton shirt and long pants if handling molten polymer.
Brdu cell proliferation kit the brdu cell proliferation assay kit is a nonisotopic assay for the in vitro quantitative detection of newly synthesized dna of actively proliferating cells. The major disadvantage of brdu staining is that the doublestranded dna blocks the access of the antibrdu antibody to brdu units. Immunofluorescent staining of cell surface antigens. Study samples from brdutreated animals were first weighed and then a volume of 50.
Assess cytotoxicity of chemotherapeutic drugs using the delfia cell proliferation assay and the spectramax i3 multimode microplate reader. Chemical product and company identification opadry ag product name. Fisher scientific 1 reagent lane fair lawn, nj 07410 for information, call. Examples safety data sheet sds msds here you can see examples of safety data sheets in some of the various languages we handle. Find msds or sds, a coa, data sheets and more information. Cyquant cell proliferation assay, for cells in culture. Consequently 5 brdu is used to study cell signaling and other processes that induce cell proliferation. Delfia cell proliferation assay on the spectramax i3 multi. The promokine edubased cell proliferation assays overcome these limitations. Brdu incorporation by cells from these animals has been detected past 70 days. Msds authoring services specialize in msds writing, creation, development and authoring. The optical properties serve as a primary calibration standard in the identification of asbestos with polarized light microscopy plm. Brdu cell proliferation assay qia58 merck millipore.
We work hard to ensure that we are fully uptodate with regulations. August 2018 colorimetric immunoassay for the quantific ation of cell proliferation, based on the measurement of brdu incorporation during dna synthesis. Safety data sheet drive xlr8 herbicide revision date. Therefore samples have to be subjected to harsh denaturing. Certificate of analysis coa product information sheet pdf. Cells can naturally incorporate the thymidine analog brdu into their cells during cell division, making this nucleoside analog.
Identification product identifier product number 7016l product name dtt dithiothreitol recommended use of the chemical and restrictions on use identified uses this product is intended for research purposes only. Bromodeoxyuridine brdu in research and to discuss the associated hazards and safe handling practices. For incorporation to occur, the brdu must be phosphorylated in the cell by thymidine kinase. This kit is stable up to 1 year after receipt, when stored as directed. Can i combine edu and brdu labeling and detection on the same sample. Bromodeoxyuridine 5bromo2deoxyuridine, brdu is an odourless white powder, a synthetic nucleoside and an analogue of thymidine. A nonradioactive alternative to the 3hthymidine incorporation assay cat. Exposure assessment of laboratory workers to hazardous. Page 310 safety data sheet according to 19072006ec, article 31 printing date 18. Identification of the substancemixture and of the companyundertaking product name. This antibody reacts with bromodeoxyuridine brdu in single stranded dna produced by partial denaturation of double stranded dna, brdu coupled to a protein carrier, as well as free brdu.
An msds material safety data sheet once was a ninesection chemical safety document detailing the toxicity, use, storage, handling and emergency procedures of hazardous substances. This incubation period is required for equilibration of dyedna binding, resulting in a stable fluorescence endpoint. Ac228590000, ac228590010, ac228590025, ac228595000, bp25081, bp2508250, bp25085, bp2508500 synonyms. Protective gloves are required when handling hot polymer. These products are carbon oxides co, co2, and other toxic compounds nitrogen oxides, isocyanate vapors. Bromodeoxyuridine competes with thymidine for incorporation into dna, resulting in dna. The resulting dna can be used in conjunction with brdu monoclonal antibody for the immunohistochemical analysis of cell proliferation. Bromodeoxyuridine brdu is a white crystalline watersoluble compound that is frequently used in cancer treatmentresearch for inducing increased susceptibility to radiation therapy, and as a topical antiviral treatment. Opadry ag colorcon 415 moyer boulevard west point, pa 19486 usa e. Apoptosis, dna damage and cell proliferation kit staining protocol. Therefore, samples have to be subjected to harsh denaturing. Hazardous ingredient ww% cas number pinumber 1017 manufacturers name chemco manufacturing. Therefore, samples have to be subjected to harsh denaturing conditions resulting in degradation of the structure of the specimen. The cyquant cell proliferation assay requires minimal handson time.
Once thawed, the reagent is stable at 4c for one year. As a msds service provider, we are specialists in ghs. Using the timeresolved detection mode of the spectramax i3 multimode reader allows for greater sensitivity than other fluorescencebased cell proliferation methods. Material safety data sheet msds for nonfat dry milk i. Safety data sheet northwest missouri state university. Bromodeoxyuridine brdu bromodeoxyuridine, also known as brdu mw 307. Identification of the substancemixture and of the companyundertaking 1. All potentially contaminated animal carcasses, tissue samples, bedding, other. Bromodeoxyuridine brdu bromodeoxyuridine, also known as brdu mw307. Brdu cell proliferation assay this proliferation assay is a nonisotopic. Background the halogenated pyrimidine thymidine analog bromodeoxyuridine brdu is incorporated into newly synthesized dna strands of s phase cells and is.
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